NECAT Beamline

The Northeastern Collaborative Access Team (NE-CAT) facility at the Advanced Photon Source at Argonne National Laboratory is managed by Cornell University and consists of seven member institutions:

  • Columbia University
  • Cornell University
  • Harvard University
  • Memorial Sloan-Kettering Cancer Center
  • Massachusetts Institute of Technology
  • Rockefeller University
  • Yale University.

    • Primary funding for this project comes from the National Center for Research Resources (NCRR), a division of the National Institutes of Health (NIH). Additional financial support for NE-CAT comes from the member institutions.

    Chemistry Laboratory

     

    NE-CAT maintains a chemistry laboratory for use by its resident staff members and beam line users adjacent to the Sector 24-ID beam lines. The laboratory is fully equipped for protein crystallization and crystal soaking experiments. Chemicals normally used are readily available as well as routine chemical equipment such as analytical balances, a pH meter, an ultrasound dismembrator, a table-top refrigerated shaker, a high-speed refrigerated Ependorf centrifuge, a UV/Vis spectrophotometer, and two high-power stereo zoom microscopes. In addition, there is a Agilent 2100 Bioanalyzer available for fast and precise characterization of molecular weights and concentrations of proteins and DNA, a dynamic light scattering apparatus which can be used to provide unique insights into the behavior of biomolecules in solution by measuring the hydrodynamic radius and polydisersity of macromolecules, and an AKTA/FPLC system housed in a temperature controlled 4 degree Centigrade box for high- throughput automated analytical and preparative protein purification. Two temperature controlled incubators are available, at 4 and 18 degrees Centigrade. There is also a small -35 degree Centigrade refrigerator available for storage of samples as well as a 9x 10 foot walk in cold room maintained at 4 degrees Centigrade.
     

    © 2005 Northeastern Collaborative Access Team

    Webmaster: Cyndi Salbego