NECAT Home Page

The Northeastern Collaborative Access Team (NE-CAT) facility at the Advanced Photon Source at Argonne National Laboratory is managed by Cornell University and consists of seven member institutions:

Columbia University

Cornell University

Harvard University

Memorial Sloan-Kettering Cancer Center

Massachusetts Institute of Technology

Rockefeller University

Yale University.

Primary funding for this project comes from the National Center for Research Resources (NCRR), a division of the National Institutes of Health (NIH). Additional financial support for NE-CAT comes from the member institutions.

The following structures have been solved using data from NE-CAT's beamlines.

Rotavirus SA11 VP1 / RNA Complexes	SAM-dependent methyltransferase from Bacteroides vulgatus ATCC 8482	Quinolinate synthase from Pyrococcus furiosus
PurLQS Thermotoga maritima	TRAPP Subassembly Activating the Rab Ypt1p	VapBC-5 from Mycobacterium tuberculosis	Peptidoglycan Glycosyltransferase Domain from Aquifex aeolicus
Putative Transglycosylase from Caulobacter crescentus	Nup107/Nup133	HIV Vif BC-box in Complex with Human ElonginB and ElonginC	NF-κB Inhibitor A20
B-subunit of the DNA gyrase from Myxococcus xanthus	RecA-ssDNA/dsDNA	Anisomycin resistant 50S Ribosomal Subunit	Platinum-DNA Adduct Bound to Deoxyguanosine
Human Factor VIII	PCNA12 Dimer from Sulfolobus solfataricus	Self-spliced Group II Intron	Prosubtilisin Substrate Complex of Subtilisin
Pyruvate Kinase M2	Vitronectin/Urokinase/Urokinase Receptor complex	L7/L12 stalk of 50S Large Ribosomal Subunit	Hepatitis C Virus IRES Subdomain IIa
CNK/HYP Dimer	Thiamin Monophosphate Kinase Aquifex aeolicus	Bacterial Carboxysome Cell	PDCD4 MA3 Domains
Ankyrin Repeat Domain of TRPV6	Oligogalacturonate lyase (VPA0088) from Vibrio Parahaemolyticus	DR_0571 Protein from Deinococcus radiodurans	HTH-type Transcriptional Regulator AcrR
EGFR - Kinase Domain	Thiamin Binding Protein	S. cerevisiae Dcn1	Metal Binding Domain of NikR
Human BPTF PHD Finger	PCNA123 Heterotrimer	Violacein Biosynthetic Enzyme VioE	Bacillus stearothermophilus UvrA
Methanocaldococcus jannaschii PurP	Pyrococcus furiosusPurP	C-Terminal Kinase Domain of P90 2 Ribosomal S6 Kinase 2	DegS, a PDZ Protease
Human Intrinsic factor: Cobalamin complex	DnaB Helicase	HMPD Decarboxylase	Inner Kinetochore Subunit Cep3p
Rebeccamycin Biosynthetic Enzyme RebC	Nucleoporin Nic96	\|Rad4 Nucleotide Excision Repair Protein	AMP-activated Protein Kinase
Pyrrolysyl-tRNA Synthetase	ADH Synthase	Lowe Syndrome Protein OCRL	C. crescentus SspB Ortholog
Yeast Sec13/31 Vertex Element	Yeast Fatty Acid Synthase	Minimal Pluripotent DNA Ligase	Phi29 DNA Polymerase
At1g77540-Coenzyme A Complex	Human Mcl-1 in Complex with Bim BH3	SCF^Cdc4 Dimer	Focal Adhesion Kinase
Non-covalent Complex Between Human SUMO-1 and Human Ubc9	Fbw7-Skp1-Cyclin E Complex	FH2 domain of Daam1	DNA Ligase Bound to Nicked DNA-Adenylate
Herpesvirus-encoded Cysteine Protease	Monobody MBP-74/Maltose Binding Protein Fusion Complex	Lung Cancer-Derived EGFR	Death Domain Containing Protein CRADD
Inosine 5'-monophosphate cyclohydrolase Methanobacterium thermoautotrophicum	Rab GTPase Sec4p	Peptidoglycan Glycosyltransferase	Amicyanin
Q7WAF1 BORPA from Bordetella parapertussis	4-Hydroxybutyrate Coenzyme A Transferase	Q9HYQ7 PSEAE from Pseudomonas aeruginosa	Escherichia coli N⁵-carboxyaminoimidazole Ribonucleotide Mutase
Aromatic Amine Dehydrogenase	Synthetic Human Lysozyme	Eurkaryotic RNA Exosome	Hydroxylase-Regulatory Protein Complex
T4 RNA Ligase 2	TmYaaDE	Thermotoga maritima Formylglycinamide Ribonucleotide Amidotransferase	Human Senp2 in Complex with PreSUMO-2
HTH-type Transcriptional Regulator yddM	FeeM, an N-Acyl Amino Acid Synthase	RNAase II	Arabidopsis thaliana Double Bond Reductase (at5G16970)
DNA polymerase III alpha subunit Thermus aquaticus	Glycoprotein B from Herpes Simplex virus 1	P52G Mutant of Amicyanin	Self-inhibited Thrombin
Active Chimera of (S)-Mandelate Dehydrogenase	Ankyrin Repeat Domain - TRPV2 Ion Channel	BMP2-induced 3-kb Gene Protein	tRNA Splicing Endonuclease
Mouse Pyrimidine 5'-Nucleotidase Type 1	Arabidopsis thaliana At1g77540	DNA Glycosylase	BPTF PHD Finger of NURF
Cbf5-Nop10-Gar1 Complex	Rb C-terminal Domain bound to E2F1-DP1	5'-Deoxy-5'-Methylthioadenosine Phosphorylase II	Ubiquitin-Conjugating Enzyme E2
RNAase A	Exocyst Subunit Exo70p	Escherichia coli ThiFS	Arsenical Resistance Protein
Human Deoxycytidine Kinase	Type IIA DNA Topoisomerase	Fe(II) HppE	Skp1-Skp2-Cks1-p27^Kip1 Complex
pH Domain of SKAP55	Eschrichia coli Uridine Phosphorylase	Succinylglutamate Desuccinylase Escherichia coli	Toxoplasma gondii Adenosine Kinase
3-Hydroxyanthranilate-3,4-dioxygenase	SWI2/SNF2 Chromatin-Remodeling Domain	14-3-3	Sspb Heterodimer
Ubiquitin Hydrolase UCH-L3	Bacillus subtilis TenI	Bacillus subtilis TenA	Trichomonas vaginalis Purine Nucleoside Phosphorylase
Hypothetical Protein (Tm1631) Thermotoga Maritima	Permutated Arc Repressor	Sspb-Rsea Complex	Human Tub Protein (Isoform A)
Protein-primed DNA Polymerase of Bacteriophage Phi29	Bacterial Nucleoside Transporter Tsx	Sspb Disulfide	Hypothetical Protein Vc1974 from Vibrio Cholerae
Cytochrome c and Cytochrome c Peroxidase	Achaeoglobus fulgidus tRNA Nucleotidyltransferase	Ytnj from Bacillus subtilis	Phage Twort Group I Ribozyme Product Complex
Long-Chain Fatty Acid Transporter FadL	Thiazole Synthase/Sulfur Carrier Protein Complex from Bacillus subtilis	Aminoimidazole Riboside Kinase from Salmonella enterica	Lysine5,6-aminomutase
T. maritima S-adenosylmethionine Decarboxylase	Bni1p Formin Homology-2 domain	a/bSerine Hydrolase (Ydr428C)	B. subtilis PurS
Protein Conducting Channel	Purine 2'-deoxyribosyltransferase	E. coli AMP Nucleosidase	Nogo-66 Receptor (NgR)
Nickel-Responsive Transcription Factor NikR	Semaphorin-3A Receptor binding Module	Human phosphopantothenoylcysteine (PPC) Decarboxylase	YaaE
Pvl ArgDC	ThiO	Human Phosphopantothenoylcysteine Synthetase	Large ribosomal E site Complexes

Recent Highlights

08/19/2008

July-August status report now available.

7/07/2008

June status report now online.

06/24/2008

NE-CAT Hires Three New User Support Staff (More Info)

06/12/2008

May status report is now available.

05/06/2008

NE-CAT organizes a successful workshop titled “Software for Challenging Cases in Macromolecular Crystallography” at APS User Week 2008. A photo of the organizers and speakers is shown. Presentations can be found here.

4/04/2008

Featured Publication

Crystal Structure of a Self-Spliced Group ll Intron

Navtej Toor, Kevin S. Keating, Sean D. Taylor, and Anna Marie Pyle

Science 4 April 2008, Vol. 320. No. 5872, pp. 77-82

Click here for full article

04/02/2008

Agenda now available for NE-CAT workshop "Software for Challenging Cases in Macromolecular Crystallography" to be held May 6 as part of User Week 2008. See agenda.

04/01/2008

With the success users have had using the MD2 Microdiffractometer on 24-ID-E, NE-CAT is acquiring a second MD2 for 24-ID-C, delivery October 2008

2/27/2008

NE-CAT hosting the workshop “WK04, Software for Challenging Cases in Macromolecular Crystallography” to be held May 6, 2008 at APS User Week. See the APS User Meeting Website

1/22/2008

Modified ALS sample placement robotic system installed on 24-ID-C beamline.

11/16/2007

Resource Advisory Committee Meeting Held

NE-CAT Director Steven Ealick Provides Introductory Overview (see additional pic )

10/01/2007

24-ID-E equipped with a MD-2 Microdiffractometer has been declared operational and accepting General User proposals for the 2008-1 run cycle. (See pic)

09/04/2007

The move of the 8-BM Bending Magnet Beamline to 24-BM is in progress. Shown is the installation of the detector support frame.

6/08/2007

16x16x300 micron crystal irradiated at 5 different positions using a 20 micron beam with the MD2 microdiffractometer to obtain a complete data set with a radiation-damage sensitive crystal. (See pic)

4/13/2007

First Data Frames Obtained Using the MD2 Microdiffractometer (see additional pictures)

03/21/2007

MD2 Microdiffractometer Installed on 24-ID-E beamline

02/16/2007

First Data Taken on Phase 2, 24-ID-E Beamline

12/15/2006

MD2 Microdiffractometer being bench tested (shown with covers removed). See December Status update for more details.

11/06/2006

Phase 2 User Control Area Instrumented (rose color panel area). Phase 1 control area in foreground. User common area in background.

10/19/2006

Phase 2 installation nearing completion with installation of ADSC Q315 Detector.

10/01/2006

NECAT ceases 8-BM user operations in preparation for moving the 8-BM Beamline to 24-BM. See September 2006 Report.

8/08/2006

Phase 2 End Station Instrumentation Being Installed. See Status Report August 2006

July 12, 2006

APS verifies that the Phase 2 end station enclosure meets the radiation standards and grants approval for NE-CAT to begin building out the end station.

5/30/2006

Obtained “first light” (monoenergetic X-rays) from Phase 2 Monochromator. See Status Report May 2006

5/22/2006

Phase 2 Horizontal Focusing Mirror and Photon Shutter Installed

5/22/2006

Phase 2 Vertical Focusing Mirror in Place.