T. thermophilus Argonaute Mutant Proteins

PDB codes:

3HJF E546 mutant complexed with DNA guide strand and 15-nt RNA target strand

3HK2 N478 mutant complexed with DNA guide strand and 19-nt RNA target strand

3HM9 complexed with DNA guide strand and 19-nt RNA target strand

3HO1 N546 mutant complexed with DNA guide strand and 12-nt RNA target strand

3HVR complexed with DNA guide strand and 19-nt RNA target strand with two Mg2+ at the cleavage site

3HXM with guide DNA and target RNA duplex containing two mismatches

Reference:

Wang, Y., Juranek, S., Li, H., Sheng, G., Wardle, G. S., Tuschl, T., and Patel, D. J. (2009) Nucleation, propagation and cleavage of target RNAs in Ago silencing complexes, Nature 461, 754-761. PubMed

Beamline: 24-ID-C