Crystal structure of the Neisseria gonorrhoeae MtrD inner membrane multidrug efflux pump.
Publication Type:Journal Article
Source:PLoS One, Volume 9, Issue 6, p.e97903 (2014)
Keywords:Amino Acid Sequence, Bacterial Proteins, Binding Sites, Crystallography, X-Ray, Membrane Proteins, Membrane Transport Proteins, Models, Molecular, Molecular Sequence Data, Neisseria gonorrhoeae, Protein Binding, Protein Conformation, Sequence Alignment
<p>Neisseria gonorrhoeae is an obligate human pathogen and the causative agent of the sexually-transmitted disease gonorrhea. The control of this disease has been compromised by the increasing proportion of infections due to antibiotic-resistant strains, which are growing at an alarming rate. The MtrCDE tripartite multidrug efflux pump, belonging to the hydrophobic and amphiphilic efflux resistance-nodulation-cell division (HAE-RND) family, spans both the inner and outer membranes of N. gonorrhoeae and confers resistance to a variety of antibiotics and toxic compounds. We here report the crystal structure of the inner membrane MtrD multidrug efflux pump, which reveals a novel structural feature that is not found in other RND efflux pumps. </p>