Preliminary crystallographic analysis of mouse Elf3 C-terminal DNA-binding domain in complex with type II TGF-beta receptor promoter DNA.
Publication Type:
Journal ArticleSource:
Acta Crystallogr Sect F Struct Biol Cryst Commun, Volume 65, Issue Pt 12, p.1261-3 (2009)Keywords:
Animals, Base Sequence, Binding Sites, Cloning, Molecular, Crystallography, X-Ray, DNA, DNA Primers, DNA-Binding Proteins, Mice, Polymerase Chain Reaction, Promoter Regions, Genetic, Protein Conformation, Protein Serine-Threonine Kinases, Receptor, Transforming Growth Factor-beta Type II, Receptors, Transforming Growth Factor beta, Recombinant Proteins, Transcription FactorsAbstract:
<p>Ets proteins are transcription factors that activate or repress the expression of genes that are involved in various biological processes, including cellular proliferation, differentiation, development, transformation and apoptosis. Like other Ets-family members, Elf3 functions as a sequence-specific DNA-binding transcriptional factor. A mouse Elf3 C-terminal fragment (amino-acid residues 269-371) containing the DNA-binding domain has been crystallized in complex with mouse type II TGF-beta receptor promoter (TbetaR-II) DNA. The crystals belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 42.66, b = 52, c = 99.78 A, and diffracted to a resolution of 2.2 A.</p>
Detector:
Q315
Beamline:
24-ID-C