Structural basis for activation of the autoinhibitory C-terminal kinase domain of p90 RSK2.

Publication Type:

Journal Article

Authors:

Malakhova, Margarita; Tereshko, Valentina; Lee, Sung-Young; Yao, Ke; Cho, Yong-Yeon; Bode, Ann; Dong, Zigang

Source:

Nat Struct Mol Biol, Volume 15, Issue 1, p.112-3 (2008)

Keywords:

Crystallography, X-Ray, Enzyme Activation, Models, Molecular, Protein Conformation, Ribosomal Protein S6 Kinases, 90-kDa

Abstract:

<p>The X-ray structure at 2.0-A resolution of the p90 ribosomal S6 kinase 2 C-terminal kinase domain revealed a C-terminal autoinhibitory alphaL-helix that was embedded in the kinase scaffold and determines the inactive kinase conformation. We suggest a mechanism of activation through displacement of the alphaL-helix and rearrangement of the conserved residue Glu500, as well as the reorganization of the T-loop into the active conformation.</p>