Structural insights into the role of rRNA modifications in protein synthesis and ribosome assembly.
Publication Type:Journal Article
Source:Nat Struct Mol Biol, Volume 22, Issue 4, p.342-344 (2015)
Keywords:Crystallography, X-Ray, Models, Molecular, Protein Biosynthesis, Protein Structure, Tertiary, Ribosomes, RNA, Bacterial, RNA, Messenger, RNA, Ribosomal, RNA, Transfer, Thermus thermophilus
<p>We report crystal structures of the Thermus thermophilus ribosome at 2.3- to 2.5-Å resolution, which have enabled modeling of rRNA modifications. The structures reveal contacts of modified nucleotides with mRNA and tRNAs or protein pY, and contacts within the ribosome interior stabilizing the functional fold of rRNA. Our work provides a resource to explore the roles of rRNA modifications and yields a more comprehensive atomic model of a bacterial ribosome. </p>