Total chemical synthesis of biologically active vascular endothelial growth factor.
Publication Type:Journal Article
Source:Angew Chem Int Ed Engl, Volume 50, Issue 35, p.8029-33 (2011)
Keywords:Amino Acid Sequence, Chromatography, High Pressure Liquid, Crystallography, X-Ray, Dimerization, Mass Spectrometry, Molecular Sequence Data, Peptides, Protein Structure, Tertiary, Vascular Endothelial Growth Factor A
<p>Efficient access: the 204-residue covalent-dimer vascular endothelial growth factor with full mitogenic activity was prepared from three unprotected peptide segments by one-pot native chemical ligations. The covalent structure of the synthetic VEGF was confirmed by precise mass measurement, and the three-dimensional structure of the synthetic protein was determined by high-resolution X-ray crystallography.</p>